| 张扬,黄友塔,王彦,张志强,于妍妍,邱维宝.基于环阵超声的高精度微量移液平台*[J].,2025,44(1):113-119 |
| 基于环阵超声的高精度微量移液平台* |
| An annular array based platform for High-precision low-volume acoustic liquid transfer |
| 投稿时间：2023-09-22 修订日期：2024-12-26 |
| 中文摘要: |
| 非接触式超声移液技术在药物开发、合成生物学、分子诊断等生物医学领域具有广阔的应用潜力。目前基于单阵元聚焦换能器的超声移液技术，因换能器制作工艺简单及能量大等优势得到了广泛的应用。然而基于单阵元换能器的超声移液方式因为换能器焦距固定需要机械移动换能器实现不同高度液体的转移，导致移液效率较低、通量低。环阵换能器可以采用电子聚焦的方式实现焦点在轴向上动态调节，能够克服单阵元换能器焦距固定的缺点，有望提高超声移液效率。因此，本文自主设计并制作了基于FPGA的五通道高精度超声信号发射系统，采用定制的五阵元环阵超声换能器搭建了一套基于环阵超声的高精度超声移液平台。该移液平台可以实现超声焦点动态精准调节，并在不同焦距情况下实现纳升级液滴精准转移，可以有效提高超声移液效率和通量，具有重要的应用价值和前景。 |
| 英文摘要: |
| Non-contact ultrasonic liquid handling technology has great potential applications in biomedical fields such as drug development, synthetic biology, and molecular diagnostics. Currently, single-element focused transducer has been widely used in the ultrasonic liquid handling technology due to its advantages of simple fabrication process and high energy output. However, the single-element transducer-based ultrasonic liquid handling method requires mechanical movement of the transducer to achieve transfer of liquids at different heights due to the fixed focal length of the transducer, resulting in low efficiency and low throughput. An annular array transducer can dynamically adjust the focus along the axial direction using electronic focusing, which overcomes the drawback of the fixed focal length of single-element transducer and is expected to improve the efficiency of ultrasonic liquid handling. Therefore, in this study, we designed and fabricated a five-channel high-precision ultrasound signal transmission system based on FPGA, and built a high-precision ultrasound liquid handling platform with a custom-designed five-element ultrasonic transducer. Experimental results demonstrate that the proposed liquid handling strategy can achieve dynamic and precise adjustment of ultrasound focus, and accurately transfer nanoliter-level liquid droplets at different focal lengths, improving the efficiency and throughput of ultrasonic liquid handling. It shows significant application value and promising prospects. |
| DOI：10.11684/j.issn.1000-310X.2025.01.011 |
| 中文关键词: 电子聚焦，环阵超声换能器，FPGA，超声移液 |
| 英文关键词: Electronic Focusing, Annular array, FPGA, Acoustic Liquid Transfer |
| 基金项目:合成生物学自动化铸造平台关键技术研发(2018YFA0902900) |
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| 摘要点击次数: 6 |
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